Melan A Specific antibody (MSVA-900M)

• Catalog Number: orb2276540
• Category: Antibodies
• Description: Melan A antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Biorbyt-900M
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG2a, kappa
• Immunogen: Recombinant full-length human MART-1 protein
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: Q16655
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen
• Note: For research use only
• Application notes: Positive Control: Skin: Virtually all melanocytes should show a strong Melan A- immunostaining including a weak to moderate staining in melanocytic dendrites. Negative Control: Kidney: Melan A staining must be absent in all cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Melan A staining is absent in the kidney.

Skin: In the skin, a strong Melan-A immunostaining is seen in melanocytes.

Skin Malignant melanoma with strong Melan A positivity in all tumor cells.