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| Catalog Number | orb764231 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to MEK-2 (phospho-Thr394) |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC-P, IP, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MEK2 around the phosphorylation site of Thr394. AA range:261-310 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 44kD |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-MAP2K2 antibody, anti-MEK2 antibody, anti-MKK Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western Blot analysis of various cells using Phospho-MEK-2 (T394) Polyclonal Antibody diluted at 1:2000.
Western Blot analysis of VEC cells using Phospho-MEK-2 (T394) Polyclonal Antibody diluted at 1:2000.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK2 (Phospho-Thr394) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from ovary cancer, using MEK2 (Phospho-Thr394) Antibody. The lane on the right is blocked with the phospho peptide.
Phospho-MEK2 (Thr394) Rabbit pAb [orb2715908]
ELISA, IHC-P, IP, WB
Human, Mouse, Rat
Polyclonal
Unconjugated
100 μl, 50 μl, 20 μl
