MED25 Antibody

• Catalog Number: orb1270916
• Category: Antibodies
• Description: MED25 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This MED25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids from the N-terminal region of human MED25.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 78 kDa
• Target: MED25
• UniProt ID: Q71SY5
• NCBI: Q71SY5
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Mediator of RNA polymerase II transcription subuni
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western blot analysis in mouse kidney tissue lysates (35 ug/lane).

Western blot analysis in MCF-7 cell line lysates (35 ug/lane).This demonstrates thdetected the MED25 protein (arrow).

MED25 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.