MED25 Antibody

• Catalog Number: orb1259131
• Category: Antibodies
• Description: MED25 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human MED25 (NP_112235.2).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 78kDa
• Target: MED25
• UniProt ID: Q71SY5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: Mediator of RNA polymerase II transcription subuni
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using MED25 antibody (orb1259131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunofluorescence analysis of U-2 OS cells using MED25 Polyclonal Antibody (orb1259131) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.