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MDH1 Rabbit Polyclonal Antibody

Catalog Number: orb6403

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb6403
CategoryAntibodies
DescriptionMDH1 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Canine, Equine, Gallus, Porcine, Rabbit
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human MDH1 (265-334/334aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW36 kDa
TargetMDH1
UniProt IDP40925
RRIDAB_10927042
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesCytosolic malate dehydrogenase; Malate dehydrogena
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NoteFor research use only
Expiration Date12 months from date of receipt.
MDH1 Rabbit Polyclonal Antibody

Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-DMT1 antibody (orb6403), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

MDH1 Rabbit Polyclonal Antibody

Blank control: Molt-4. Primary Antibody (green line): Rabbit Anti-MDH1 antibody, dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 0.2 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

MDH1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (MDH1) Polyclonal Antibody, Unconjugated (orb6403) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

MDH1 Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Heart tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Mouse Kidney tissue lysates, Lane 4: Mouse Stomach tissue lysates, Lane 5: Rat Heart tissue lysates, Lane 6: Rat Cerebrum tissue lysates, Lane 7: Rat Stomach tissue lysates, Lane 8: Human HL-60 cell lysates, Lane 9: Human MOLT4 cell lysates, Primary: Anti-MDH1 (orb6403) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kDa, Observed band size: 36 kDa.

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