MDH1 Protein, Human, Recombinant (His)

Catalog Number: orb1961362

Catalog Number	orb1961362
Category	Proteins
Description	Malate Dehydrogenase, Cytoplasmic (MDH1) is an enzyme which belongs to the MDH Type 2 sub-family of LDH/MDH superfamily. MDH1 is involved in the Citric Acid Cycle that catalyzes the conversion of Malate into Oxaloacetate (using NAD+) and vice versa. MDH1 should not be confused with Malic Enzyme, which catalyzes the conversion of Malate to Pyruvate, producing NADPH. MDH1 also participates in Gluconeogenesis, the synthesis of Glucose from smaller molecules. Pyruvate in the mitochondria is acted upon by Pyruvate Carboxylase to form Pxaloacetate, a Citric Acid Cycle intermediate. In order to transport the Oxaloacetate out of the Mitochondria, Malate Dehydrogenase reduces it to Malate, and it then traverses the inner Mitochondrial membrane. Once in the cytosol, the Malate is oxidized back to Oxaloacetate by MDH1. Finally, Phosphoenol-Pyruvate Carboxy Kinase (PEPCK) converts Oxaloacetate to Phosphoenol Pyruvate.
Tag	C-6xHis
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Protein Sequence	Ser2-Ala334
UniProt ID	P40925
MW	36 KDa (reducing condition)
Expression System	E. coli
Biological Origin	Human
Expression Region	Ser2-Ala334
Storage	-20°C
Note	For research use only
Expiration Date	6 months from date of receipt.

Purity:	≥90% as determined by SDS-PAGE
Source:	This protein contains the human MDH1(Ser2-Ala334) was fused with the C-terminal His Tag and expressed in E. coli.