MDC1 antibody

• Catalog Number: orb148035
• Category: Antibodies
• Description: Mouse monoclonal to MDC1 (ATTO-594). MDC1, mediator of DNA damage checkpoint protein 1, plays a role in checkpoint mediated cell cycle arrest in response to DNA damage, within S phase and G2/M. It is also thought to act as a scaffold protein during recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage that are marked by phosphorylation of histone H2A. X on S139..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: P2B11
• Tested applications: ICC, WB
• Reactivity: Bovine, Human, Mouse, Primate
• Isotype: IgG1
• Immunogen: GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus
• Concentration: 1 mg/ml
• Dilution range: WB (1:2000), ICC/IF (1:100)
• Conjugation: ATTO-594
• MW: 184kDa
• Target: MDC1
• Entrez: 240087
• UniProt ID: Q5PSV9
• NCBI: NP_001010833.2
• Storage: Conjugated antibodies should be stored at 4°C
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Alternative names: Nuclear factor with BRCT domains1 antibody, mediat
• Note: For research use only
• Application notes: 0.5 µg/ml of SMC-197 was sufficient for detection of MDC1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.
• Expiration Date: 12 months from date of receipt.