MCU Rabbit Polyclonal Antibody

• Catalog Number: orb317655
• Category: Antibodies
• Description: MCU Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Human, Porcine, Rabbit, Sheep
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MCU (251-351//351aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 40 kDa
• Target: MCU
• UniProt ID: Q8NE86
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Alternative names: MCU_HUMAN; C10orf42; Ccdc109a; Coiled-coil domain-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Yu, Ziqing et al. The mechanical effects of CRT promoting autophagy via mitochondrial calcium uniporter down-regulation and mitochondrial dynamics alteration J. Cell. Mol. Med., (2019)

PubMed: 30938090

Applications

IF, WB

Reactivity

Canine

Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MCU) Polyclonal Antibody, Unconjugated (orb317655) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.