MCT1 Rabbit Polyclonal Antibody (BF488)

• Catalog Number: orb1581610
• Category: Antibodies
• Description: MCT1 Rabbit Polyclonal Antibody (BF488)
• Target: SLC16A1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: BF488
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Porcine, Rabbit
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human MCT1 (251-350/500aa)
• Tested applications: FC, IF
• Dilution range: Flow-Cyt=1μg/Test, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: monocarboxylate transporter 1; Malignant T cell am
• Note: For research use only

Blank control (Black line): U937 (Black). Primary Antibody (green line): Rabbit Anti-MCT1/AF488 antibody (orb1581610), Dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-AF488. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-MCT1 Antibody, conjugated (orb1581610) 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.