MCP3 Rabbit Polyclonal Antibody

• Catalog Number: orb13567
• Category: Antibodies
• Description: MCP3 Rabbit Polyclonal Antibody
• Target: CCL7
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Rat
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from mouse MCP-3 (24-97/97aa)
• RRID: AB_10920093
• MW: 11 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: C-C motif chemokine 7; CCL7; CCL7_HUMAN; Chemokine
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Longobardi, L. et al. Role of the C-C chemokine receptor-2 in a murine model of injury-induced osteoarthritis Osteoarthritis Cartilage, 25, 914-925 (2017)

PubMed: 27856294

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (MCP3) Polyclonal Antibody, Unconjugated (orb13567) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.