CCL2 Antibody

SKU: orb345137

Description

MCP1 antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:10,000, WB: 1:1000
• Reactivity: Human
• Application Notes:
  This purified antibody has been tested in western blotting and suitable in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 11,025 Da in size corresponding to the mature human MCP-1 protein by western blotting in appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human MCP-1 protein produced in E.coli.
• Target: CCL2
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human MCP-1 protein. A BLAST analysis was used to suggest cross-reactivity with MCP-1 from human sources based on 100% homology with the immunizing sequence. Based on 98 to 100% homology, there is a chance of cross-reactivity to MCP-1 from chimpanzee, orangutan, macaque, and baboon. Cross-reactivity with MCP-1 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: None. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-MCP-1 Antibody, CCL2, C-C motif chemokine 2, Monocyte Chemotactic and Activating Factor, MCAF, Small-inducible cytokine A2, SCYA2, Monocyte chemotactic protein 1, Monocyte secretory protein JE, HC11

Anti-human MCP-1 by western blot shows detection of recombinant human MCP-1 raised in E.coli. Recombinant (0.1 µg, expect ~8.6 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Biorbyt's Anti-Human MCP-1. After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20000 in blocking buffer (p/n orb348637) for 30 min at RT.

Quick Database Links

Gene Symbol

CCL2

UniProt

P13500

RefSeq (Protein):NP_002973.1