MCM7 Antibody

• Catalog Number: orb1252231
• Category: Antibodies
• Description: MCM7 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MCM7/1466
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: Amino acids 195-319 were used as the immunogen for the MCM7 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: IF: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTThe stated application concentrations are suggested starting amounts. Titration of the MCM7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MCM7
• UniProt ID: P33993
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: DNA replication licensing factor MCM7, CDC47 homol
• Note: For research use only
• Application notes: IF: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTThe stated application concentrations are suggested starting amounts. Titration of the MCM7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human 1) HeLa, 2) Raji and 3) HepG2 cell lysate with MCM7 antibody (clone MCM7/1466). Expected molecular weight: 80-90 kDa.

IHC testing of FFPE human tonsil with MCM7 antibody (clone MCM7/1466). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free MCM7 Antibody (clone MCM7/1466). Confirmation of Integrity and Purity of the Antibody.