MCM7 Antibody

• Catalog Number: orb234307
• Category: Antibodies
• Description: MCM7 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human MCM7 recombinant protein (Position: D526-V719). Human MCM7 shares 94% amino acid (aa) sequence identity with mouse MCM7.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry, 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 81308 MW
• UniProt ID: P33993
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: DNA replication licensing factor MCM7;3.6.4.12;CDC
• Note: For research use only
• Application notes: WB: The detection limit for MCM7 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U937 cells using anti-MCM7 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of A431 cells using anti-MCM7 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of MCM7 using anti-MCM7 antibody.Lane 1:human HeLa cell;2:human K562 cell;3:human Jurkat cell;4:human MCF-7 cell;5:rat C6 cell;6:mouse Neuro-2a cell.

IF analysis of MCM7 using anti-MCM7 antibody.MCM7 was detected in paraffin-embedded section of human colon cancer tissues.

IF analysis of MCM7 using anti-MCM7 antibody. MCM7 was detected in paraffin-embedded section of human colon cancer tissues.

IF analysis of MCM7 using anti-MCM7 antibody.MCM7 was detected in immunocytochemical section of U20S cells.

IHC analysis of MCM7 using anti-MCM7 antibody. MCM7 was detected in a paraffin-embedded section of Mouse Testis tissue.

IHC analysis of MCM7 using anti-MCM7 antibody. MCM7 was detected in a paraffin-embedded section of Rat Intestine tissue.

IHC analysis of MCM7 using anti-MCM7 antibody. MCM7 was detected in a paraffin-embedded section of Human Lung Cancer tissue.

IHC analysis of MCM7 using anti-MCM7 antibody. MCM7 was detected in immunocytochemical section of A549 cell.