MCM7 Antibody

• Catalog Number: orb1252146
• Category: Antibodies
• Description: MCM7 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MCM7/1468
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: A human partial recombinant protein corresponding to amino acids 195-319 was used as the immunogen for the MCM7 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Immunofluorescence: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Titering of the MCM7 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MCM7
• UniProt ID: P33993
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: DNA replication licensing factor MCM7, CDC47 homol
• Note: For research use only
• Application notes: Immunofluorescence: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Titering of the MCM7 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Analysis of HuProt(TM) microarray containing more than 19000 full-length human proteins using MCM7 antibody (clone MCM7/1468). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC testing of FFPE human tonsil with MCM7 antibody (clone MCM7/1468). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free MCM7 Antibody (clone MCM7/1468). Confirmation of Integrity and Purity of the Antibody.