MCM3 antibody

• Catalog Number: orb1473938
• Category: Antibodies
• Description: Mouse monoclonal antibody to MCM3
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein of human MCM3. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/2000), FC (1/10 - 1/50)
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MCM3
• Entrez: 4172, 17215
• UniProt ID: P25206, P25205
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: DNA replication licensing factor MCM3; DNA polymer
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MCM3 expression in CHO (A), NIH3T3 (B), Hela (C), HepG2 (D), C2C12 (E), PC12 (F) whole cell lysates. (Predicted band size: 90 kD; Observed band size: 100 kD)

Flow cytometric analysis of Hela cells using Anti-MCM3 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.