MCM2 Antibody

• Catalog Number: orb2635769
• Category: Antibodies
• Description: MCM2 is a component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. [UniProt]
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 650-750 was used as the immunogen for the MCM2 antibody.
• UniProt ID: P49736
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the MCM2 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: MCM2/3678
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This MCM2 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human colon carcinoma tissue with MCM2 antibody (clone MCM2/3678). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with MCM2 antibody (clone MCM2/3678). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free MCM2 antibody (clone MCM2/3678) as confirmation of integrity and purity.