MCM2 Antibody (C-term)

• Catalog Number: orb1927953
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB25168
• Tested applications: IHC-P, WB
• Predicted Reactivity: Other
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:2000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 101896 Da
• Target: This MCM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 649-677 amino acids from the C-terminal region of human MCM2.
• UniProt ID: P49736
• NCBI: NP_004517.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: DNA replication licensing factor MCM2, Minichromos
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MCM2 Antibody (C-term) in HL-60 cell line lysates (35 ug/lane). MCM2 (arrow) was detected using the purified Pab.

MCM2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCM2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-MCM2 Antibody (C-term) at 1:2000 dilution. Lane 1: Jurkat whole cell lysates. Lane 2: MCF-7 whole cell lysates. Lane 3: NIH/3T3 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 102 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.