MCL1 Antibody

• Catalog Number: orb1239463
• Category: Antibodies
• Description: MCL1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Mcl-1 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human Mcl-1.The immunogen is located within amino acids 220 - 270 of Mcl-1.
• Concentration: 1 mg/mL
• Dilution range: Mcl-1 antibody can be used for detection of isoforms Mcl-1L and Mcl-1ES by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 22, 39 kDa Observed: 39 kDa
• Target: MCL1
• UniProt ID: Q07820
• NCBI: NP_068779
• Storage: Mcl-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Mcl-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Mcl-1 Antibody: TM, EAT, MCL1L, MCL1S, Mcl-1, BCL2
• Note: For research use only
• Application notes: Mcl-1 antibody can be used for detection of isoforms Mcl-1L and Mcl-1ES by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Mcl-1 in Raji cell lysate with Mcl-1 antibody at 0.5 µg/mL.

Immunocytochemistry staining of Raji cells using Mcl-1 antibody at 2 µg/mL.

Immunofluorescence of Mcl-1 in Raji cells with Mcl-1 antibody at 10 µg/mL.