MCL1 Antibody

• Catalog Number: orb215887
• Category: Antibodies
• Description: MCL1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human MCL1 recombinant protein (Position: M1-R350). Human MCL1 shares 82% and 78% amino acid (aa) sequences identity with mouse and rat MCL1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 37337 MW
• UniProt ID: Q07820
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Induced myeloid leukemia cell differentiation prot
• Note: For research use only
• Application notes: WB: The detection limit for MCL1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of MCL1 using anti-MCL1 antibody.Lane 1:recombinant human MCL1 protein 0.5 ng.

WB analysis of MCL1 using anti-MCL1 antibody.Lane 1:rat spleen tissue; 2:human HepG2 cell; 3:human MCF-7 cell; 4:human COLO320 cell.

IHC analysis of MCL1 using anti-MCL1 antibody. MCL1 was detected in a paraffin-embedded section of rat intestine tissue.

IHC analysis of MCL1 using anti-MCL1 antibody. MCL1 was detected in a paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of MCL1 using anti-MCL1 antibody. MCL1 was detected in a paraffin-embedded section of mouse intestine tissue.