mCherry Antibody

SKU: orb153320

Description

Goat polyclonal antibody to mCherry (Cherry fluorescent protein). mCherry protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma.

Images & Validation

• Tested Applications: IEM, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
• Reactivity: Other
• Application Notes:
  The antibody solution should be gently mixed before use.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Antigen: Purified recombinant peptide produced in E. coli.. Antigen Sequence: MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK
• Target: Red Fluorescent Protein
• Purification: Epitope affinity purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 20% glycerol and 0.05% sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.

Western blot analysis of HEK293 cell line lysate using mCherry antibody.

Confocal immunofluoroscence analysis of HEK293 cells using mCherry antibody.

Quick Database Links

Gene Symbol

Red Fluorescent Protein

References

Ji-Il Kim, Kent Imaizumi, Mayuri Vijay Thete, Zuzana Hudacova, Ovidiu Jurjuţ, Neal D Amin, Grégory Scherrer, Sergiu P Paşca Human assembloid model of the ascending neural sensory pathway Nature, (2025)

PubMed: 40205039

Applications

IHC

Reactivity

Human