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Catalog Number | orb1526449 |
---|---|
Category | Antibodies |
Description | MCEF Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP |
Reactivity | Human |
Immunogen | between 50 and 100 |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended. A302-538A has not performed satisfactorily when used for WB of MCEF in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of MCEF.; IP - 5 - 15 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | MCEF |
UniProt ID | Q9UHB7 |
NCBI | NP_055238.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | AF5Q31; Protein AF-5q31; MCEF; major CDK9 elongati Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human MCEF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MCEF antibody orb1526449 used for IP at 6 µg per reaction.
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