MBP tag Rabbit Polyclonal Antibody

SKU: orb2088

Description

MBP tag Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, ELISA=1:1000-5000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000, Flow-Cyt=1ug/Test, ICC/IF=1:100-500
• Reactivity: All
• Predicted Reactivity: E. coli

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from E. coli Maltose Binding Protein (1-100/396aa)
• Target: malE
• Molecular Weight: 44 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Tb; b4034; ECK4026; MALE_ECOLI; malE; MMBP; Maltodextrin-binding protein; Maltose-binding protein (MBP); MALE_ECO57; c5004;

Sample: Lane 1: MBP tagged cytochrome C (Full Length) Protein at 4 ug Lane 2: MBP tagged caspase 3 p17 (143-213) Protein at 4 ug Primary: Anti-MBP tag (orb2088) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60-70 kD Observed band size: 62 kD.

Sample: Lane 1: MBP-Tagged Fusion Protein at 300 ng, Primary: Anti-MBP tag (orb2088) at 1/20000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 55 kDa, Observed band size: 60 kDa.

Sample: Lane 1: MBP-tagged fusion protein Overexpression E.coli Lysate at 4 ug, Primary: Anti-MBP tag (orb2088) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 70 kD, Observed band size: 70 kD.

Quick Database Links

Gene Symbol

malE