MBP tag Rabbit Polyclonal Antibody

• Catalog Number: orb2088
• Category: Antibodies
• Description: MBP tag Rabbit Polyclonal Antibody
• Target: malE
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: All
• Predicted Reactivity: E. coli
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from E. coli Maltose Binding Protein (1-100/396aa)
• MW: 44 kDa
• Tested applications: ELISA, WB
• Dilution range: WB=1:500-2000, ELISA=1:1000-5000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MBP-tag; MBP; MMBP; Maltose Binding Protein; Malto
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: MBP tagged cytochrome C (Full Length) Protein at 4 ug Lane 2: MBP tagged caspase 3 p17 (143-213) Protein at 4 ug Primary: Anti-MBP tag (orb2088) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60-70 kD Observed band size: 62 kD.

Sample: Lane 1: MBP-Tagged Fusion Protein at 300 ng, Primary: Anti-MBP tag (orb2088) at 1/20000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 55 kDa, Observed band size: 60 kDa.

Sample: Lane 1: MBP-tagged fusion protein Overexpression E.coli Lysate at 4 ug, Primary: Anti-MBP tag (orb2088) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 70 kD, Observed band size: 70 kD.