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MBP Control protein

SKU: orb345959

Description

MBP Control protein

Images & Validation

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Tested ApplicationsSDS-PAGE, WB
Application Notes
Maltose-Binding Protein is suitable for use as a control in many assays including affinity purification, western blot and other biological assays. Optimal concentration should be determined by the researcher. Expect MW of MBP~51kDa consisting of multiple cloning sites from vector (MCS) and the MBP; molecular weight of MBP at ~40kDa. Maltose-Binding Protein has been tested in SDS-page and western blot.

Key Properties

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Expression SystemE. coli
ConjugationUnconjugated
PurityMaltose binding protein is expressed in Escherichia coli and chromatographically purified using amylose resin.

Storage & Handling

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StorageStore vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration5.0 mg/mL
Expiration Date6 months from date of receipt.
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

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control protein, MBP, Maltose Binding Protein

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol