VISA Rabbit Polyclonal Antibody

• Catalog Number: orb580779
• Category: Antibodies
• Description: Rabbit polyclonal antibody to VISA
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Bovine, Mouse
• Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human VISA
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 56kDa
• Target: MAVS
• UniProt ID: Q2HWT5
• Protein Sequence: Synthetic peptide located within the following region: ETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGH
• NCBI: NP_065797
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: IPS1, VISA, IPS-1, CARDIF
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Application: IHC, Species+tissue/cell type: 293T cells transfected with 3FLAG-VISA, Primary antibody dilution: 1:500, Secondary antibody: Anti-rabbit-Alexa 488, Secondary antibody dilution: 1:500.

Sample Type: 1: 20 ug HEK293T no transfection, 2: 20 ug HEK293T 3Flag-MAVS/VISA, Primary Antibody dilution: 1:1000, Secondary Antibody: Anti-rabbit HRP, Secondary Antibody dilution: 1:1000.

WB Suggested Anti-VISA Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:12500, Positive Control: Transfected 293T.