Matriptase Rabbit Polyclonal Antibody

• Catalog Number: orb11026
• Category: Antibodies
• Description: Matriptase Rabbit Polyclonal Antibody
• Target: TMPRSS6
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human Matriptase (11-100/855aa)
• UniProt ID: Q8IU80
• RRID: AB_10747548
• MW: 94 kDa
• Tested applications: ICC
• Dilution range: ICC/IF=1:50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: transmembrane protease, serine 6; 1300008A22Rik; I
• Research Area: Cancer Research, TMPRSS2
• Note: For research use only

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Matriptase) polyclonal Antibody, Unconjugated (orb11026) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Jurkat (Human) CellLysate at 40 ug, Primary: Anti-Matriptase (orb11026) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 94 kD, Observed band size: 125 kD.