MARS Antibody

• Catalog Number: orb1523854
• Category: Antibodies
• Description: MARS Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 650 and 700
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: MARS
• UniProt ID: P56192
• NCBI: NP_004981.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: cytosolic methionyl-tRNA synthetase; SPG70; MTRNS;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse MARS by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-MARS antibody orb1523854 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human MARS by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-MARS antibody orb1523854 used for IP at 6 µg/mg lysate.

Detection of human MARS by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MARS used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB