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MARCH8 Rabbit Polyclonal Antibody

SKU: orb1445

Description

MARCH8 Rabbit Polyclonal Antibody

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200
ReactivityRat
Predicted ReactivityBovine, Equine, Gallus, Human, Mouse, Porcine, Rabbit, Sheep

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human MARCH8 (101-200/291aa)
TargetMARCHF8
Molecular Weight33 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

c MIR; c-MIR; Cellular modulator of immune recognition; E3 ubiquitin protein ligase MARCH8; E3 ubiquitin-protein ligase MARCH8; MARCH 8; MARCH VIII; MARCH-VIII; MARCH8; MARH8_HUMAN; Membrane associated RING CH protein VIII; Membrane associated ring finger(C3HC4) 8; Membrane associated RING finger protein 8; Membrane-associated RING finger protein 8; Membrane-associated RING-CH protein VIII; MIR; RING finger protein 178; RNF178.

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MARCH8 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (MARCH8) Polyclonal Antibody, Unconjugated (orb1445) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

UniProt Details

No UniProt data available

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

MARCH8 Rabbit Polyclonal Antibody (orb1445)

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Available Sizes

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50 μl
$ 250.00
100 μl
$ 390.00
200 μl
$ 470.00
DispatchUsually dispatched within 5-10 working days
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