MARCH7 Rabbit Polyclonal Antibody

• Catalog Number: orb1444
• Category: Antibodies
• Description: MARCH7 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Sheep
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MARCH7/Axotrophin (31-130/704aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 78 kDa
• Target: MARCHF7
• UniProt ID: Q9H992
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: AXO; AXOT; Axotrophin; E3 ubiquitin-protein ligase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (orb1444) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat uterus), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (orb1444) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug, Lane 2: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-MARCH7 (orb1444) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 78 kD, Observed band size: 78/74 kD.