MAPK15 Rabbit Polyclonal Antibody

• Catalog Number: orb582755
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MAPK15
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Predicted Reactivity: Rat
• Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MAPK15
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 60kDa
• Target: MAPK15
• UniProt ID: Q8TD08
• Protein Sequence: Synthetic peptide located within the following region: GHDPAEHESPRAAKNVPRQNSAPLLQTALLGNGERPPGAKEAPPLTLSLV
• NCBI: NP_620590
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: ERK7, ERK8
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Lanes: Lane 1: 50 ug untransfected HEK-293T lysate, Lane 2: 50 ug MAPK15 transfected HEK-293T lysate, Primary Antibody dilution: 1:2000, Secondary Antibody: Anti-rabbit-HRP, Secondary Antibody dilution: 1:1000, Gene Name: MAPK15.

Sample Type: HA-MAPK15 transfected HeLa cells, Primary Antibody dilution: 1:200, Secondary Antibody: Anti-rabbit-Alexa Fluor 555, Secondary Antibody dilution: 1:800, Color/Signal Descriptions: A: Anti-HA-Alexa Fluor 488 B: MAPK15 C: DAPI D: Merge, Gene Name: MAPK15.

WB Suggested Anti-MAPK15 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: MCF7 cell lysate.