MAP3K6 Antibody

SKU: orb1248601

Description

MAP3K6 Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Human, Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 140kDa band observed in Rat Heart lysates (calculated MW of 143kDa according to Human NP_004663.3 and Rat NP_001101379.1). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RAILAQRAGSTP
• Target: MAP3K6
• Molecular Weight: Approx 140kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

MAP3K6, mitogen-activated protein kinase kinase kinase 6, ASK2, MAPKKK6, MGC125653, MGC20114, OTTHUMP00000005063, apoptosis signal-regulating kinase 2

orb1248601 (0.3 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

MAP3K6

UniProt

O95382

RefSeq (Protein):NP_004663.3