MAP3K13/LZK Rabbit Polyclonal Antibody

SKU: orb185458

Description

MAP3K13/LZK Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Primate

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MAP3K13/LZK (801-900/966aa)
• Target: Map3k13
• Molecular Weight: 108 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

EC 2.7.11.25; Leucine zipper bearing kinase; Leucine zipper bearing kinase; Leucine zipper-bearing kinase; LZK; M3K13_HUMAN; MAP3K13; MEKK13; MGC133196; Mitogen activated protein kinase kinase kinase 13; Mitogen-activated protein kinase kinase kinase 13; Mixed lineage kinase; MLK; OTTHUMP00000210397.

Sample: K562 Cell (Human) Lysate at 40 ug, Primary: Anti-MAP3K13/LZK (orb185458) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 108 kD, Observed band size: 108 kD.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MAP3K13) Polyclonal Antibody, Unconjugated (orb185458) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Quick Database Links

Gene Symbol

Map3k13

UniProt

O43283