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Catalog Number | orb154569 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to MAP2K2 which belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family. It highly expressed in the neonatal brain. It catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MAP2K2 activates the ERK1 and ERK2 MAP kinases. Genetic variation in MAP2K2 are a cause of cardiofaciocutaneous syndrome. |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Dilution range | ELISA: 1:128000, WB: 0.1-0.3 μg/ml |
Conjugation | Unconjugated |
MW | 44,4 |
Target | MAP2K2 / MEK2 |
Entrez | 5605 |
Protein Sequence | DLKMLTNHTFIKRS |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti MAP2K2 antibody, anti mitogen-activated prote Read more... |
Note | For research use only |
Application notes | WB: Approx 48kDa band observed in lysates of cell lines Daudi, Jurkat, K562 and Molt4 (calculated MW of 44.4kDa according to NP_109587.1). Recommended concentration: 0.1-0.3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Daudi (Lane 1), Jurkat (Lane 2), K562 (Lane 3) and Molt4 (Lane 4) lysates using MAP2K2 antibody
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, WB | |
Human, Mouse | |
Monoclonal | |
Unconjugated |
IHC-P, WB | |
Drosophila, Gallus, Hamster, Mouse, Rabbit, Rat, Xenopus | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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