MAP2 Rabbit Polyclonal Antibody

SKU: orb1294406

Description

Rabbit polyclonal antibody to MAP 2

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:500-1:1000, Immunofluorescence 1:200-1:300, Immunohistochemistry (Paraffin) 1:100-1:150
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the N-terminus region.
• Target: MAP2
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

All lanes: Anti-MAP2 antibody at 1/500 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294406 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294406 anti-MAP2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescent analysis. Sample: primary cortical neurons, MAP2 antibody (orb1294406): 1-200, Anti-rabbit 488: 1-500, Fixed: 4% paraformaldehyde at RT for 20 min.

Immunohistochemical analysis of paraffin embedded Human brain and mouse tissue labeling MAP2 antibody with orb1294406 at 1/100.

Immunohistochemical analysis of paraffin embedded Human brain and mouse tissue labeling MAP2 antibody with orb1294406 at 1/100.

Quick Database Links

Gene Symbol

MAP2