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Catalog Number | orb1274543 |
---|---|
Category | Antibodies |
Description | MAP1LC3A/MAP1LC3B Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Porcine, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide of human LC3B |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 16kDa |
Target | MAP1LC3A/MAP1LC3B |
UniProt ID | Q9GZQ8 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | MAP1LC3B Antibody: LC3B, ATG8F, MAP1LC3B-a, MAP1A/ Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using LC3B antibody (orb1274543) at 1:1000 dilution. Both C6 cells and NIH/3T3 cells were treated by Chloroquine (50 μM) at 37 ℃ for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Western blot analysis of extracts from normal (control) and LC3B knockout (KO) 293T cells, using LC3B antibody (orb1274543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using LC3B antibody (orb1274543) at dilution of 1:100. Hela cells were treated by Chloroquine (50 μM) for 20 hours (left). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using LC3B antibody (orb1274543) at dilution of 1:100. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours (left). Blue: DAPI for nuclear staining.
IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P | |
Bovine, Canine, Gallus, Human, Porcine, Rat, Sheep |
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