MAP1LC3A/MAP1LC3B Antibody

• Catalog Number: orb1254777
• Category: Antibodies
• Description: MAP1LC3A/MAP1LC3B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human LC3A / LC3B (NP_073729.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 14kDa, 16kDa
• Target: MAP1LC3A/MAP1LC3B
• UniProt ID: Q9H492, Q9GZQ8
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (orb1254777) at 1:1000 dilution. Hela cells were treated by Chloroquine (50 μM) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded human breast cancer using LC3A/LC3B antibody (orb1254777) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using LC3A/LC3B antibody (orb1254777) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human mammary cancer using LC3A/LC3B antibody (orb1254777) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using LC3A/LC3B antibody (orb1254777) at dilution of 1:100. Hela cells were treated by Chloroquine (50 μM) for 20 hours (left). Blue: DAPI for nuclear staining.