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MAP1LC3A Rabbit Polyclonal Antibody

Catalog Number: orb10971

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb10971
CategoryAntibodies
DescriptionMAP1LC3A Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P
Predicted ReactivityBovine, Gallus, Porcine, Rat
ReactivityHuman, Mouse
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human MAP1LC3A (75-121/121aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2μg/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW14 kDa
TargetMAP1LC3A
UniProt IDQ9H492
RRIDAB_10753303
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesATG8E; Autophagy-related protein LC3 A; Autophagy-
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NoteFor research use only
Expiration Date12 months from date of receipt.
MAP1LC3A Rabbit Polyclonal Antibody

Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-MAP1LC3A antibody (orb10971), Dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 70% ethanol (Overmight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min at -20°C. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

MAP1LC3A Rabbit Polyclonal Antibody

Blank control: RAW264.7. Primary Antibody (green line): Rabbit Anti-MAP1LC3A antibody (orb10971), Dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

MAP1LC3A Rabbit Polyclonal Antibody

Blank control: RAW264.7. Primary Antibody (green line): Rabbit Anti-MAP1LC3A antibody (orb10971), Dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

  • Cleaved LC3A Antibody [orb1933527]

    ICC,  IF,  IHC-P,  WB

    Rat, Zebrafish

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • Phospho-LC3C(S12) Antibody [orb1931205]

    DOT,  WB

    Mouse, Rat, Zebrafish

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • LC3 Rabbit Polyclonal Antibody [orb500748]

    WB

    Bovine, Equine, Human, Porcine, Rat

    Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl
  • Cleaved LC3A Antibody [orb1787989]

    IF,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • MAP1LC3A antibody(N-term) [orb1925347]

    IHC-P,  WB

    Zebrafish

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl