MANF Antibody

SKU: orb1239620

Description

MANF Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-MANF antibody (orb1239620) was raised against a peptide corresponding to 12 amino acids near the amino terminus of human MANF. The immunogen is located within the first 50 amino acids of MANF.
• Target: MANF
• Molecular Weight: Predicted: 20kDObserved: 26 kD
• Purification: MANF Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: MANF Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

MANF Antibody: ARP, ARMET, ARP, Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich protein

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: MANF orb1239620, (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MANF recombinant protein per lane. Antibodies: MANF orb1239620 (Lane 1: 0.125 µg/mL, Lane 2: 0.25 µg/mL and Lane 3: 0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 55kD.

Western Blot Validation in Rat Brain Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: MANF orb1239620 (A: 1 µg /mL and B: 2 µg /mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of MANF in Human Brain Tissue. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-MANF antibody (orb1239620) at 2.5 µg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of MANF in Human Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling MANF with orb1239620 at 20 µg /mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Induced Expression Validation of MANF in Mouse C17.2 Neural Stem Cells (Almutawaa et al., 2014). Concentration- and time-dependent effects of Valproic acid (VPA) on MANF at 48 h examined by Western blot analysis with anti-MANF antibodies. MANF was markedly increased 48h after VPA treatment. Lanes 1–4: control, 0.5 mM VPA, 1 mM VPA, 3 mM VPA.

Quick Database Links

Gene Symbol

MANF

UniProt

P55145

RefSeq:P55145