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Catalog Number | orb1259585 |
---|---|
Category | Antibodies |
Description | MANBA Antibody |
Target | MANBA |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 18-300 of human MANBA (NP_005899.3). |
UniProt ID | O00462 |
MW | Observed: 101kDa |
Tested applications | IHC, WB |
Application notes | WB: 1:200 - 1:2000IHC: 1:50 - 1:200 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Beta-mannosidase, 3.2.1.25, Lysosomal beta A manno Read more... |
Note | For research use only |
Western blot analysis of extracts of various cell lines, using MANBA antibody (orb1259585) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
IH, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Equine, Mouse, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |