MAN2A1 antibody

• Catalog Number: orb19722
• Category: Antibodies
• Description: Goat polyclonal antibody to MAN2A1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml
• Conjugation: Unconjugated
• MW: 131
• Target: MAN2A1
• Entrez: 4124
• Protein Sequence: KISSDIKSQNR
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti MAN2A1 antibody, anti mannosidase, alpha, cla
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 150kDa band observed in Human Placenta lysates (calculated MW of 131kDa according to NP_002363.2). In transfected HEK293 transiently expressing MAN2A1 a band of approx. 98kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19722 (0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing Man2A1 (RC220186) and probed with orb19722 (mock transfection in first lane), tested by Origene.