MAGEB3 Rabbit Polyclonal Antibody

SKU: orb100761

Description

MAGEB3 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Equine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MAGEB3 (201-300/346aa)
• Target: MAGEB3
• Molecular Weight: 39 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cancer/testis antigen family 3 member 5; CT3.5; MAGB3_HUMAN; MAGE B3 antigen; MAGE-B3 antigen; MAGEB3; melanoma antigen family B, 3; Melanoma associated antigen B3; Melanoma-associated antigen B3.

Sample: Brain (Mouse) Lysate at 40 ug, Primary: Anti-MAGEB3 (orb100761) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39 kD, Observed band size: 39 kD.

Tissue/cell: Human melanoma tumor, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-MAGEB3 Polyclonal Antibody, Unconjugated (orb100761) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: mouse testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-MAGEB3 Polyclonal Antibody, Unconjugated (orb100761) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: Rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-MAGEB3 Polyclonal Antibody, Unconjugated (orb100761) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

MAGEB3

UniProt

O15480