MAG Rabbit Polyclonal Antibody

SKU: orb11014

Description

MAG Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MAG-a/b (501-582/582aa)
• Target: MAG
• Molecular Weight: 67 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

GMA; MAG; MAG_HUMAN; Myelin associated glycoprotein; Myelin-associated glycoprotein; S MAG; Siglec 4a; Siglec-4a; SIGLEC4A.

Blank control: SH-SY5Y. Primary Antibody (green line): Rabbit Anti-MAG antibody (orb11014), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: U251 Cell Lysate at 30 ug, Primary: Anti-MAG (orb11014) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 67 kD, Observed band size: 67 kD.

Quick Database Links

Gene Symbol

MAG

UniProt

P20916