Macrophage Inflammatory Protein 1 gamma Rabbit Polyclonal Antibody

• Catalog Number: orb13568
• Category: Antibodies
• Description: Macrophage Inflammatory Protein 1 gamma Rabbit Polyclonal Antibody
• Target: Ccl9
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from mouse CCL9 (22-122/122aa)
• UniProt ID: P51670
• RRID: AB_10749282
• MW: 13 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MIP-1 gamma; Macrophage inflammatory protein 1-gam
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin embedded mouse skin probed with Rabbit Anti-Macrophage Inflammatory Protein 1 gamma Polyclonal Antibody (orb13568) at 1:50 for two hours at room temperature (green channel).

Tissue/Cell: mouse brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MIP-1 gamma Polyclonal Antibody, Unconjugated (orb13568) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.