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Description
Research Area
Pharmacology & Drug Discovery
Images & Validation
−
| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:1,000 - 1:20,000, WB: 1:500 - 1:3,000 |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with methylated lysine conjugated KLH. |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using methylated lysine peptide coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG. Reactivity is specific for methylated lysine residues (mono, di). The cross reactivity with tri-methyl lysine has not been tested. There are no cross reactions with acetylated proteins. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 50% (v/v) Glycerol; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0 |
| Concentration | 250 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−Dimethylysine antibody, Methyl lysine antibody, N epsilon dimethyl lysine antibody, N epsilon mono methyllysine antibody, N epsilon trimethyl lysine antibody, N(epsilon) trimethyl lysine antibody, Trimethyl lysine antibody
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Lysine Methylated Antibody (orb420312)
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