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Lysine Methylated Antibody

SKU: orb420312

Description

Lysine Methylated antibody

Images & Validation

Tested ApplicationsELISA, WB
Dilution rangeELISA: 1:1,000 - 1:20,000, WB: 1:500 - 1:3,000
Application Notes
This affinity-purified antibody is suitable for use in ELISA, immunohistochemistry, western blotting and immunoprecipitation assays. Although not tested, this antibody is likely functional in RIA, and flow cytometry.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenThis affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with methylated lysine conjugated KLH.
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using methylated lysine peptide coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG. Reactivity is specific for methylated lysine residues (mono, di). The cross reactivity with tri-methyl lysine has not been tested. There are no cross reactions with acetylated proteins.
ConjugationUnconjugated

Storage & Handling

StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLiquid (sterile filtered)
Buffer/Preservatives0.01% (w/v) Sodium Azide
Concentration250 µg/ml
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

Dimethylysine antibody, Methyl lysine antibody, N epsilon dimethyl lysine antibody, N epsilon mono methyllysine antibody, N epsilon trimethyl lysine antibody, N(epsilon) trimethyl lysine antibody, Trimethyl lysine antibody

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Quality Guarantee

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Lysine Methylated Antibody (orb420312)

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