LYPLA3 Rabbit Polyclonal Antibody

SKU: orb185108

Description

LYPLA3 Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cell Biology, Lipid Metabolism

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human LYPLA3 (101-200/412aa)
• Target: PLA2G15
• Molecular Weight: 43 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

1 O acylceramide synthase; 1-O-acylceramide synthase; ACS; DKFZp564A0122; Group XV phospholipase A2; GXVPLA 2; GXVPLA2; LCAT like lysophospholipase; LCAT-like lysophospholipase; LLPL; LPLA 2; LPLA2; LYPLA 3; Lysophospholipase 3(lysosomal phospholipase A2); Lysophospholipase 3; Lysosomal phospholipase A2; OTTHUMP00000174859; PAG15_HUMAN; phospholipase A2, group XV; PLA2G15; UNQ341/PRO540.

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ZC3HAV1L) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Placenta tissue lysates, Lane 2: Mouse Lung tissue lysates, Lane 3: Mouse NIH/3T3 cell lysates, Lane 4: Human Jurkat cell lysates, Lane 5: Human HepG2 cell lysates, Lane 6: Human HeLa cell lysates, Primary: Anti-LYPLA3 (orb185108) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kDa, Observed band size: 48 kDa.

Quick Database Links

Gene Symbol

PLA2G15

UniProt

Q8NCC3