Lyn Rabbit Polyclonal Antibody

• Catalog Number: orb6338
• Category: Antibodies
• Description: Lyn Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Lyn (201-300/512aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 58 kDa
• Target: LYN
• UniProt ID: P07948
• RRID: AB_10920605
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Hck 2; JTK 8; JTK8; ONCOGENE LYN; Tyrosine protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Mouse Lymph node tissue lysates, Lane 2: Mouse Lung tissue lysates, Lane 3: Mouse Placenta tissue lysates, Lane 4: Rat Lymph node tissue lysates, Lane 5: Rat Placenta tissue lysates, Lane 6: Human Raji cell lysates, Primary: Anti-Lyn (orb6338) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kDa, Observed band size: 50 kDa.

Sample: SP2/0 (Mouse) Cell Lysate at 30 ug, Primary: Anti-Lyn (orb6338) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD.

Tissue/Cell: human glioma tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Lyn Polyclonal Antibody, PE conjugated 1:200, used at 1:200 dilution for 60 minutes at 37°C. Excitation wavelength: 488nm, 555nm, Emission wavelength: 578nm.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Lyn Polyclonal Antibody, Unconjugated (orb6338) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.