Lymphotoxin alpha antibody

• Catalog Number: orb613927
• Category: Antibodies
• Description: Mouse monoclonal antibody to Lymphotoxin alpha
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3F12.2D3
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: 3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTα expressed in E. coli - the hybridoma clone was selected for its ability to bind LTα3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: Lymphotoxin alpha
• UniProt ID: P01374
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: LTα3 chimeric 3F12;LT;; LTα; Tumor necrosis factor
• Note: For research use only
• Application notes: The properties of the chimieric 3F12 is equivalent to that of the original 3F12 mAb. 3F12 is able to bind to Th2 cells and additionally is can functionally block human LTα3 versus an isotype control (trastuzumab) - LTα3-induced ICAM upregulation is blocked on HUVEC (Human Umbilical Endothelial Cells) (IC50 0.04 nM) and LTα3-induced IL-8 is blocked in A549 cells. The antibody has also been used in ELISA studies to detect LT-alpha and to measure binding.
• Expiration Date: 12 months from date of receipt.