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Catalog Number | orb1671468 |
---|---|
Category | Antibodies |
Description | LTA Antibody (Biotin) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure recombinant Human TNF-beta. Anti-Human TNF-beta specific antibody was purified by affinity chromatography and then biotinylated. |
Concentration | batch dependent |
Form/Appearance | Lyophilized |
Conjugation | Biotin |
Target | LTA |
UniProt ID | P01374 |
NCBI | P01374 |
Storage | Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | Tumor Necrosis Factor-beta, TNFSF1B, Lymphotoxin-a Read more... |
Note | For research use only |
Application notes | Sandwich ELISA: To detect Human TNF-beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human TNF-beta as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human TNF-beta. Western Blot To detect Human TNF-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human TNF-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
To detect Human TNF-beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TNF-beta as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human TNF-beta.
To detect Human TNF-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human TNF-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human TNF-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human TNF-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA, IHC-P | |
Bovine, Canine, Equine, Mouse, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Biotin |
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