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| Catalog Number | orb2096 |
|---|---|
| Category | Antibodies |
| Description | LYL1 Rabbit Polyclonal Antibody |
| Target | LYL1 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Mouse, Porcine, Rat |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Purification | Affinity purified by Protein A |
| Immunogen | KLH conjugated synthetic peptide derived from human LYL1 (151-250/280aa) |
| UniProt ID | P12980 |
| MW | 31 kDa |
| Tested applications | FC |
| Dilution range | Flow-Cyt=1ug/test |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Lyl1; Lymphoblastic leukemia derived sequence 1; P Read more... |
| Research Area | Cancer Research, Epigenetics and Nuclear Signaling Read more... |
| Note | For research use only |

U-937 cells were fixed with 4% PFA for 10 min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with LYL1 Antibody (orb2096) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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