Ly6g Antibody

• Catalog Number: orb1671788
• Category: Antibodies
• Description: Ly6g Antibody
• Clonality: Recombinant
• Clone Number: RB6-8C5
• Tested applications: Depletion, FC, IHC, WB
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised in Lou-M rats using a popliteal lymph node immunisation and fusion protocol as described by Martiniello et al (1994)
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Ly6g
• UniProt ID: P35461
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Gr-1, Gr1, Ly-6G, ly-6G.1, ly6C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of mouse splenocytes using anti-Ly6G/Ly6C antibody (RB6-8C5). Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of RB6-8C5 (orb1671788) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671788- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.

Flow-cytometry using the Anti-Ly6G/Ly6C antibody RB6-8C5. Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r version of RB6-8C5 (orb1671788 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.