Ly6G antibody (Biotin)

• Catalog Number: orb756847
• Category: Antibodies
• Description: Rat monoclonal antibody against Ly6G conjugated to Biotin
• Clonality: Monoclonal
• Clone Number: RB6-8C5
• Tested applications: FC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Mouse
• Isotype: Rat IgG2b
• Immunogen: Murine granulocytes
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 2-5 μg/ml.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: Ly6G
• Entrez: 546644
• UniProt ID: A0A087WQF5
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Gr1 antibody, anti- Gr-1 antibody, anti- Ly-6
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 2-5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of murine myeloid Ly6G positive cells stained using anti-Ly6G (RB-8C5) Biotin antibody (concentration in sample 1.0 μg/ml, Streptavidin APC, red-filled) from murine myeloid cells unstained by primary antibody (Streptavidin APC, black-dashed) in flow cytometry analysis.

Flow cytometry surface staining pattern of murine peritoneal fluid cell suspension stained using anti-murine Ly6G (RB6-8C5) Biotin antibody (concentration in sample 1.0 μg/ml, Streptavidin APC).